WebJan 13, 2024 · The most promising LC3-binding SUPR peptide accessed the cytosol at low micromolar concentrations as measured by chloroalkane penetration assay (CAPA) and inhibited starvation-mediated GFP-LC3 puncta formation in a … WebDec 17, 2024 · Herein, we demonstrate that by the addition of a chloroalkane tag on the BRD4 specific degrader, MZ1, cell permeability can be quantified via the Chloroalkane …
(PDF) Drug interaction mapping with proximity dependent enzyme ...
WebFeb 28, 2024 · recent chloroalkane penetration assay (CAPA) is coupled with automated high content (HC) imaging, a technique that combines the advantages of fl uorescence microscopy and fl ow cytometry. WebFeb 15, 2024 · We have applied the chloroalkane penetration assay, which exclusively reports on material that reaches the cytosol, to investigate the effects of these … cottage dreams baby bedding
Cell Penetration Profiling Using the Chloroalkane …
WebJul 21, 2024 · The chloroalkane penetration assay was used to quantify the cytosolic delivery of SAH-JGZ4 using A549 cells stably expressed Halo-GFP-Mito, the … WebOct 6, 2024 · For quantitative detection in the cytosol, the 2D chloroalkane penetration assay (CAPA) is translated to 3D deep tissue. The targeted delivery of quantum dots, … WebFeb 15, 2024 · We have applied the chloroalkane penetration assay, which exclusively reports on material that reaches the cytosol, to investigate the effects of these characteristics on the cytosolic uptake of druglike oligonucleotides. We found that base composition and base sequence had moderate effects, while length did not correlate … cottage door press bedtime songs