Webapplicable for analyses of protein-protein and protein-nucleic acid interactions14,15, proteome stress16,17, protein folding and aggregation18,19, dynamics and hydration20–22, or cell-permeability23. HaloTag fusions enable protein control in vivo24–26, including degradation27,28 or dimerization29 of proteins of interest. WebNov 22, 2024 · Here, we report the development of a protein–oligonucleotide conjugation method involving a high-affinity capture tag, HaloTag, to link proteins to DNA oligonucleotides, and its application in protein profiling, ... Then, 9 μl bait protein (pIX-His-HALO-bZIP53) produced using the TNT-coupled wheat germ extract (Promega) ...
HaloTag - Wikipedia
WebAnti-HaloTag® Monoclonal Antibody. 200μg. $ 464.00. Your price: Log in. Anti-HaloTag® Monoclonal Antibody is a mouse monoclonal antibody raised against the HaloTag® … WebHaloTag® Standard Protein. 30μg. $ 77.00. Your price: Log in. The HaloTag® Standard Protein is a 61kDa purified HaloTag®-GST fusion protein supplied at a concentration of … hs to rgb
HaloTag: A Novel Protein Labeling Technology for Cell Imaging …
WebThe development of fusion tag systems is essential for purification and analysis of recombinant proteins. The HaloTag is a 34 KDa monomeric protein derived from a … WebJan 30, 2024 · The specific and covalent labeling of the protein HaloTag with fluorescent probes in living cells makes it a powerful tool for bioimaging. However, the irreversible attachment of the probe to HaloTag precludes imaging applications that require transient binding of the probe and comes with the risk of irreversible photobleaching. Here, we … WebCLIP-tag is a modified version of SNAP-tag. It is also a self-labeling protein derived from human O 6 -alkylguanine-DNA-alkyltransferase. Instead of benzylguanine derivatives, CLIP tag is engineered to react with benzylcytosine derivatives. It can be used as a protein tag for tagging your protein of interest (POI). hst organization