Web14. júl 2024 · 3.2 对照样品中的Index 错误分配. 1、如图2a 所示,针对BGISEQ-500 测序仪,标准WGS文库构建包括主要包括以下几个步骤:1、DNA片段化;2、末端修复及添加A尾;3、indexed adapter 连接(该步骤为每个样品添加唯一index 标记);4、PCR扩增;5、单链环格式(ssCir);6、DNB 准备。 Web正如图所示,大概分为如下步骤。 用酶或者激光或者超声波将Genomic DNA或者由RNA反转组得到的双链cDNAs打断成小片段 打断是随机打断,有可能末端不平整,还需要用酶补平 补平之后,需要在3’端加A碱基 加上A之后,再加adapter 这时候,我们好像心里有那么点数,但是依然不知道adapter具体是怎么加上去的,也并不知道接头中, read1 sequncing …
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